FIG. 6.

Individually expressed leader protein affects nucleocytoplasmic traffic. HeLa-3E cells were transfected with the plasmids encoding leader proteins fused to RFP. (A) Efflux of the nuclear protein marker (3×EGFP-NLS) into the cytoplasm is visible in a proportion of cells transfected with the plasmid encoding wild-type (wt) L protein but not with the one encoding inactive C19A/C22A (Zn) mutant. Cells were stained with Hoechst 33342 DNA stain and captured 24 h posttransfection. (B) Percentage of the cells transfected with wild-type (wt) and mutant L-expressing plasmids, demonstrating 3×EGFP-NLS fluorescence in the cytoplasm. The transfected cells were incubated in the presence of 100 μM zVAD(OMe).fmk to prevent apoptosis, which could complicate interpretation of the results.