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. 2009 Jan 21;83(7):3238–3248. doi: 10.1128/JVI.01824-08

FIG. 4.

FIG. 4.

Pin1 contributes to Tax interaction with IKKγ. (A) 293T cells were cotransfected with 1.5 μg of control siRNA (lanes 1 and 2) or 1.5 μg of siRNA targeted against Pin1 (lanes 3 and 4) and FLAG-tagged Tax (lanes 2 and 4). FLAG-Tax was immunoprecipitated using anti-FLAG agarose beads. IKKγ was detected using anti-IKKγ (upper panel). Amounts of FLAG-Tax and IKKγ in the immunoprecipitate and Pin1 in the cell extract were verified using anti-FLAG, anti-IKKγ, and anti-Pin1, respectively. (B) WT MEFs (lanes 1 to 4) and Pin1 KO MEFs (lanes 5 to 8) were cotransfected with control plasmid (4 μg; lanes 1 and 5), with HA-Tax (3 μg; lanes 3, 4, 7, and 8), or with FLAG-Pin1-expressing vector (1 μg; lanes 2, 4, 6, and 8). HA-Tax was immunoprecipitated using anti-HA agarose beads. HA-Tax and IKKγ in the immunoprecipitate and Pin1 in the cell extract were detected using anti-HA, anti-IKKγ, and anti-Pin1, respectively. IB, immunoblotting; IP, immunoprecipitation; α, anti.