FIG. 2.

One-step growth kinetics. RK13 (A) or RK13-UL37 (B) cells were infected with noncomplemented (n) or phenotypically complemented (c) deletion mutants PrV-ΔUL37 and PrV-ΔUL37/UL35GFP as well as with PrV-Ka and PrV-UL35GFP at an MOI of 5. At the indicated times after infection (h p.i.), cells were harvested together with the supernatants, and progeny virus titers (PFU/ml) were determined by plaque assays on RK13-UL37 cells. Shown are mean titers and standard deviations of three independent experiments.