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. 2009 Jan 14;83(7):3389–3396. doi: 10.1128/JVI.02090-08

FIG. 5.

FIG. 5.

Nuclear translocation of incoming capsids. RK13 cells were infected at an MOI of 200 with PrV-UL35GFP (left panels), noncomplemented PrV-ΔUL37/UL35GFP (n) (middle panels), or phenotypically pUL37-complemented PrV-ΔUL37/UL35GFP (c) (right panels). Cells were fixed immediately after 1 h of virus adsorption on ice (0 min, top row) or 5 min (second row), 10 min (third row), and 1 h (bottom row) after the temperature shift to 37°C. Direct and indirect fluorescence reactions were analyzed by confocal laser-scanning microscopy. Green, pUL35-eGFP; red, MT; blue, actin. Viral capsids attached to the cell surface are marked by arrows. Bar, 10 μm.