FIG. 3.

Different antiviral efficacies of epitope-specific CD8 T-cell lines enriched in vitro from PBMCs. (A) The generated cell lines were stimulated in vitro in the presence of peptides representing epitopes in an IFN-γ ELISPOT assay. There was no difference among all lines in terms of the magnitude (SFC per million cells) or specificity (% response) of response specific for Gag (n = 14) versus Env epitopes (n = 12) and calculated as the percentage of the epitope-specific response of the overall HIV-specific CD8 T-cell response. Statistical comparisons were made using the Mann-Whitney test. (B) Example of differences in the antiviral efficacy of epitope-specific CD8 T-cell lines generated from a single subject (013646a) against X4 and R5 viruses during a 7-day period. The control uninfected CD4 T cells showed that there were no autologous viruses grown out from the tested subjects during the period of the assay. (C) Summary of data demonstrating different antiviral efficacies for Gag- and Env-specific cell lines against X4 and R5 in log units of inhibition after 7 days of culture. Inhibition was evaluated in multiple assays for each cell line at least twice (mean ± standard deviation).