FIG. 5.

Apical supernatant harvested from SARS-CoV-infected Calu-3 cells reduces the phagocytic activity of Mφ. Mφ cultured with the apical supernatants derived from mock- or SARS-CoV-infected Calu-3 cells, or RPMI 1640-10% FCS medium, for 3 days, as described for Fig. 2, were harvested, and their capacity to take up FITC-conjugated dextran sulfate was analyzed by fluorescence-activated cell sorter analysis as described in Materials and Methods. Results are representative of three independent experiments using Mφ derived from different individuals.