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. 2009 Jan 28;83(7):3187–3199. doi: 10.1128/JVI.01579-08

FIG. 4.

FIG. 4.

Dual immunogold labeling for tegument VP22 with label for TGN-46 in mid and distal axons of HSV-1-infected human DRG. Ultrathin sections were incubated with sheep polyclonal antibody against TGN-46 and with rabbit polyclonal antibody to VP22. Label for TGN-46 was detected with a 10-nm gold conjugate and for VP22 with a 15-nm gold conjugate. (A) Label for both TGN-46 (10-nm gold particles; arrow) and VP22 (15-nm gold particles; arrowhead) is associated with a tubulovesicular membrane structure partially surrounding a viral capsid in a growth cone. Inset shows higher magnification of the unenveloped capsid and label for both TGN-46 and VP22. (B) Label for both TGN-46 (arrows) and VP22 (arrowheads) is associated with vesicles surrounding fully enveloped viral capsids in a growth cone. (C) Label for TGN-46 (arrows) on enveloped viral capsids within vesicles accumulating in a growth cone. (D) Label for both TGN-46 (arrows) and VP22 (arrowheads) decorating extracellular viral particles. Bars, 200 nm.