TABLE 2.
Quantification of viral particles and vesicles carrying viral proteins in axons containing immunogold label for TGN-46, Rab3A, GAP-43, SNAP-25, and kinesin-1
| Vesicles or HSV-1 particles with label for VP16, VP22, or gB | % of viral particles or vesicles (no. marked/total) carrying indicated cellular markera
|
||||
|---|---|---|---|---|---|
| TGN-46 | Rab3A | GAP-43 | SNAP-25 | Kinesin-1 | |
| Vesicles in mid axons | 80.0 (24/30) | 50.0 (12/24) | 52.2 (12/23) | 40.0 (10/25) | 35.5 (22/62) |
| Vesicles in growth cones and varicosities | 85.7 (12/14) | 62.5 (10/16) | 65.2 (15/23) | 46.1 (6/13) | 53.6 (15/28) |
| Enveloped capsids in growth cones and varicosities | 68.0 (17/25) | 50 (5/10) | 62.5 (5/8) | 80.0 (12/15) | 72.2 (13/18) |
| Extracellular virions | 80.3 (61/76) | 35.1 (13/37) | 71.4 (15/21) | 73.7 (59/80) | 74.4 (64/86) |
a
Apart from kinesin-1 and SNAP-25, immunogold label for TGN-46, Rab3A, and GAP-43 was not observed on unenveloped capsids in axons.