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. 2009 Jan 19;83(7):3268–3275. doi: 10.1128/JVI.01849-08

FIG. 2.

FIG. 2.

The NS4A cofactor does not affect direct RNA binding by NS3. The parameters for each binding curve are listed in Table 1. In all cases, the Hill equation with a cooperativity value of n = 1 provided the best-fit curve (see Materials and Methods). In panel A, the curves are NS3 (solid circles) and NS3-4A (solid squares). In panel B, the curves are NS3-4A (solid circles), NS3-4A S1369R (solid squares), NS3-4A Y1702A (solid diamonds), and NS3-4A S1369R/Y1702A (hatched circles). In panel C, the curves are NS3-4A (solid circles), NS3-4A S1369R (solid squares), and NS3-4A M1708A (solid diamonds), NS3-4A S1369R/M1708A (hatched circles). These data are the averages of three experiments, and the error represents the standard deviation.