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Summary of RNA filter-binding data for NS3-4A variants
| Protein | Avg ± SDa
|
|
|---|---|---|
| Kd (nM) for binding to ssRNA | Maximal fraction of bound RNA | |
| His-NS3 | 1.0 ± 0.4 | 0.99 ± 0.01 |
| His-NS3-4A | 3 ± 1 | 0.99 ± 0.01 |
| NS3 | 9 ± 2 | 0.98 ± 0.02 |
| NS3-4A | 10 ± 1 | 0.84 ± 0.03 |
| NS3-4A S1369R | 10 ± 3 | 0.98 ± 0.01 |
| NS3-4A Y1702A | 13 ± 1 | 0.90 ± 0.03 |
| NS3-4A M1708A | 5 ± 1 | 0.90 ± 0.03 |
| NS3-4A S1369R/Y1702A | 2.0 ± 0.1 | 0.88 ± 0.02 |
| NS3-4A S1369R/M1708A | 7 ± 1 | 0.96 ± 0.02 |
These data are averages from three experiments.
