TABLE 2.

Summary of RNA-stimulated ATPase activities of NS3-4A variants^a

Protein	Avg ± SD			kcat/Kmd	RNA stimulation factor
	Km (μM)	Vmaxb	kcatc
NS3	0.100 ± 0.001	0.48 ± 0.03	0.80 ± 0.02	8	3.0
NS3-4A	0.0010 ± 0.0001	0.14 ± 0.01	0.14 ± 0.01	140	5.0
S1369R	0.0010 ± 0.0002	0.16 ± 0.01	0.16 ± 0.01	160	4.0
Y1702A	0.5 ± 0.1	0.10 ± 0.02	0.10 ± 0.02	0.2	2.0
M1708A	0.5 ± 0.2	0.23 ± 0.02	0.23 ± 0.02	0.5	2.0
S1369R/Y1702A	0.3 ± 0.1	0.17 ± 0.02	0.17 ± 0.02	0.6	2.0
S1369R/M1708A	0.0020 ± 0.0003	0.090 ± 0.003	0.090 ± 0.003	45	2.0

^aThese data are averages from three experiments.

^bValues are expressed in nmol of ATP hydrolyzed/min.

^cValues are expressed in nmol of ATP hydrolyzed/min·pmol of protein.

^dValues are expressed in nmol of ATP hydrolyzed/min·pmol of protein/μM RNA.