FIG. 2.
The 5′-proximal region 1 is sufficient to mediate RNA1 recruitment in yeast. Total RNA was extracted from yeast expressing RNA1 regions 1 (R1), 4 (R4), or 5 (R5) in the absence or presence of FAD692E. Total RNA (2 μg) was analyzed by Northern blotting with 32P-labeled cRNA probes corresponding to positive-strand RNA1 nt 1 to 377, nt 2287 to 2843, or nt 2847 to 3107 or 18S rRNA. The levels of positive-strand RNA1 were determined from six experiments after normalization to 18S rRNA and are expressed as the level of protein A-stimulated RNA1 accumulation relative to wild-type RNA1 (see Materials and Methods). The arrowheads denote the locations of positive-strand RNA1 fragments.