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RNA1 nt 206 to 277 are not important for RNA1 replication in yeast and Drosophila. Total RNA from yeast (A) or Drosophila cells (B) expressing protein Awt and wild-type (wt) or deletion mutants of RNA1, depicted in Fig. 6, was collected and analyzed by Northern blotting with 32P-labeled probes against RNA1 nt 2718 to 3064. The asterisk represents a background band. The ratios of negative-strand RNA1 to full-length RNA1fs levels in yeast (C) and Drosophila cells (D) are shown.
