TABLE 2.

Nucleotide biases found at nucleotide positions 979 to 1007 in plasmids and in vRNAs from rescued viruses

Nucleotide	% of nucleotides for:
	Randomized pool (at plasmid level)a	Selected clones (at vRNA level)b	Wild typeb
A	33.4	34.6	31
U	38.5	39.8	31
G	9	6.4	13.8
C	19	19.2	24.1

^aEscherichia coli DH5α was transformed with a plasmid pool for Mrdm vRNA. Plasmids for Mrdm vRNAs were recovered from 22 randomly selected colonies to determine the nucleotide sequences. The percentages of nucleotides corresponding to the nucleotide sequences at the 5′ end (positions 979 to 1007) of the M vRNAs in the selected plasmids are shown.

^bThe percentages of nucleotides at positions 979 to 1007 of M vRNAs derived from 38 plaque-purified and wild-type viruses are shown.