FIG. 6.

Kinetics of humoral response in mice immunized separately with integrating (IN⁺), nonintegrating (IN⁻), and ghost LNT-HBS vector viruses. Sera were taken at different times after a single intramuscular injection, and pools were made from all sera (n = 4) taken at a single time point. (A) Total Ig response was measured by detecting bound antibodies with biotinylated anti-mouse Ig. (B) IgG response was detected by using rat anti-mouse IgG1 and IgG2a antibodies separately conjugated to horseradish peroxidase. (C) IgM-to-IgG class shift response was measured by detecting antibodies in mouse sera with biotin-conjugated anti-mouse IgM and previously described anti-mouse IgG1 antibody. IN⁺, integration-competent virus; IN⁻, integration-deficient virus.