TABLE 2.
Mutations differentiating BoHV-1.1 Ciney and BoHV-1.2 K22 obtained by sequence alignment of PCR products in seven less conserved regions of BoHV-1 genome
| Gene (sequenced region) | Length of the sequenced region (bp) | SNP position(s) in the ORFa | Codon modification [BoHV-1.1]aa→[BoHV-1.2]aac | Mutation typeb |
|---|---|---|---|---|
| US8 (N terminal) | 258 | 55 | [TTA]Leu→[CTA]Leu | Ts S |
| US6 (N terminal) | 511 | 208 | [ACG]Thr→[GCG]Ala | Ts NS |
| 426 | [TTT]Phe→[TTC]Phe | Ts S | ||
| 462 | [ACG]Thr→[ACA]Thr | Ts S | ||
| US2 (N terminal) | 273 | 87 | [CTT]Leu→[CTC]Leu | Ts S |
| 149 | [CAC]His→[CGC]Arg | Ts NS | ||
| 169, 170 | [AAG]Lys→[GCG]Ala | Ts-Tv NS | ||
| 265 | [CGC]Arg→[TGC]Cys | Ts NS | ||
| LR ORF2 | 321 | 73 | [ACT]Thr→[GCT]Ala | Ts NS |
| UL11 | 288 | 165 | [AAA]Lys→[AAG]Lys | Ts S |
| 166, 167 | [AAA]Lys→[GGA]Gly | Ts NS | ||
| 169 | [AAC]Asn→[GAC]Asp | Ts NS | ||
| 187 | [GAC]Asp→[AAC]Asn | Ts NS | ||
| UL27 (N terminal) | 314 | 702 | [TTT]Phe→[TTC]Phe | Ts S |
| 740 | [CTG]Leu→[CCG]Pro | Ts S | ||
| UL44 (N terminal) | 280 | 144 | [GCT]Ala→[GCC]Ala | Ts S |
| 227 | [GTG]Val→[GGG]Gly | Tv NS | ||
| 303 | [CCC]Pro→[CCT]Pro | Ts S | ||
| 372 | [CCT]Pro→[CCC]Pro | Ts S |
a
SNPs selected for TaqMan genotyping assay are indicated in boldface type. ORF, open reading frame.
b
Ts, transition; Tv, transversion; S, synonymous; NS, nonsynonymous.
c
aa, amino acid.