TABLE 1.
S. pombe strains used in this study
| Straina | Genotype |
|---|---|
| 265 | h−smt0 leu1-32 ura4-D18 |
| 251 | h+ ura4-D18 leu1-32 |
| 260 | h−smt0 rad50::kanMX6 leu1-32 ura4-D18 |
| 284 | h+ rad50::kanMX6 leu1-32 ura4-D18 |
| 258 | h+ rad50-K81I leu1-32 ura4-D18 |
| 259 | h−smt0 rad50-K81I leu1-32 ura4-D18 |
| 263 | h+ rec12-152::LEU2 leu1-32 ura4-D18 |
| 264 | h−smt0 rec12-152::LEU2 leu1-32 ura4-D18 |
| 261 | h+ rad50::kanMX6 leu1-32 ura4-D18 rec12-152::LEU2 |
| 262 | h−smt0 rad50::kanMX6 leu1-32 ura4-D18 rec12-152::LEU2 |
| 266 | h+ leu1-32 ura4-D18 rec12-152::LEU2 rad50-K81I |
| 267 | h− smt0 leu1-32 ura4-D18 rec12-152::LEU2 rad50-K81I |
| 805 | h− smt0 ura4-D18 rad32-D65N |
| 814 | h+ rad32-D65N ura4-D18 |
| 810 | h−smt0 ura4-D18 rad32-D65N rad50::kan |
| 811 | h+ ura4-D18 rad32-D65N rad50::kan |
| 812 | h−smt0 ura4-D18 rad32-D65N rad50S |
| 813 | h+ ura4-D18 rad32-D65N rad50S |
| 352/354 | h−/h+ ade6-M216/ade6-M210 ura4-aim/ura4-aim ura4-D18/ura4-D18 rad50-K81I/rad50-K81I |
| 611 | h−smt0 ade6-M26 pat1-114 rec12-6HA:kanMX6† |
| 617 | h−smt0 pat1-114 ade6-M26 rec12-6HA:kanMX6 rad50-K81I |
| 649 | h−smt0 ade6-M26 rec12-6HA:kanMX6 rad50::kanMX6 pat1-114 |
| 418 | h+ mat1PD17::LEU2 leu1-32 arg6-1 |
| 417 | h−smt0 ade7-152 |
| 411 | h+ mat1PD17::LEU2 leu1-32 rad50-K81I arg6-1 |
| 412 | h−smt0 ade7-152 rad50-K81I |
| 407 | h+ mat1P::LEU2 leu1-32 rad50::kanMX6 arg6-1 |
| 408 | h−smt0 rad50::kanMX6 ade7-152 |
| 421 | h+ smt0 lys7-1 leu1-32 |
| 422 | h+ mat1PD17::LEU2 leu1-32 ade2-17 ura2-10 |
| 405 | h−smt0 lys7-1 leu1-32 rad50::kanMX6 |
| 406 | h+ mat1PD17::LEU2 ade2-17 ura2-10 leu1-32 rad50::kanMX6 |
| 415 | h−smt0 rad50-K81I lys7-1 leu1-32 |
| 416 | h+ mat1PD17::LEU2 rad50-K81I leu1-32 ade2-17 ura2-10 |
| 157 | h−smt0 ade6-M26 pat1-114 |
| h+ ade6-M26 pat1-114 leu1-32 | |
| h+ ade6-M26 rad50-kanMX6 pat1-114 leu1-32 | |
| h+ ade6-M26 rad32::ura4 pat1-114 leu1-32 | |
| h+ ade6-M26 rad50-K81I pat1-114 leu1-32 | |
| 207/209 | h−/h−smt0/smt0 pat1-114/pat1-114 ade6-M210/ade6-M216 ura4-aim/ura4-aim ura4-D18/ura4-D18 |
| 203/205 | h−/h−smt0/smt0 pat1-114/pat1-114 ade6-M210/ade6-M210 ura4-aim/ura4-aim ura4-D18/ura4-D18 rad50::kanMX6/rad50::kanMX6 |
| 148/152 | h−/h−pat1-114/pat1-114 ade6-M210/ade6-M216 ura4-aim/ura4-aim ura4-D18/ura4-D18 rad50-K81I rad50-K81I |
| 808 | h−smt0 pat1-114 rad32-D65N rec12-6HA:kan ade6-M26 |
| 834 | h−smt0 pat1-114 rad32-D65N rec12-6HA:kan ade6-M26 rad50::kan |
| 832 | h−smt0 pat1-114 rad32-D65N rec12-6HA:kan ade6-M26 rad50S |
| 824 | h−smt0 ctp1::kan pat1-114 rec12-6HA:kan |
a
Numbers are from the strain collection of E. Hartsuiker. The rec12-6HA-kanMX strains were created in the lab of K. Ohta. Other unpublished strains/constructs for this study were created by E. Hartsuiker.