FIG. 1.

Heparanase and CD69 transcript induction kinetics and Pol II recruitment. (A to D) Heparanase and CD69 mRNA kinetics were measured by TaqMan real-time PCR of cDNA prepared from resting (NS or 0) Jurkat T cells and cells stimulated with PI for the times indicated. mRNA levels are expressed as n-fold changes relative to those of NS samples. For actinomycin D (Act D) experiments, NS cells or cells stimulated for 24 h with PI were treated with actinomycin D for either 0.5 or 2 h prior to the 24-h time point. Panels A and B show the transcript induction kinetics of CD69 and heparanase, while panels C and D show actinomycin data for CD69 and heparanase, respectively. Data shown are the mean ± the standard error of three replicate experiments. The asterisk refers to the number of copies of heparanase detected per microgram of total RNA. (E to G) RNA Pol II ChIP assays were performed with Jurkat T cells that were either left NS or stimulated with PI for the times indicated. The names and positions of the primer sets are indicated beneath the graphs. ChIP data are shown for the CD69 gene promoter (E), the heparanase gene promoter (F), and across the CD69 gene region (G). The results represent either the mean ± the standard error of three independent experiments (E and F) or a representative experiment from three replicates (G).