FIG. 2.

Chromatin accessibility and core histone occupancy changes upon induction of the heparanase and CD69 genes. (A and B) CHART assays were performed with Jurkat T cells left NS or stimulated for 4 h with PI either across the heparanase gene promoter (A) or on the CD69 gene promoter (B). The names and positions of the primer sets are indicated beneath the graphs. Data are graphed as percent accessibility with respect to the sample minus the accessibility agent. Data are pooled from three independent experiments and show the mean ± the standard error. (C to G) ChIP assays were performed with Jurkat T cells either left NS or stimulated with PI for the times indicated. ChIP was conducted with either anti-H3 antibody (C to E) or anti-H4 antibody (F and G). Data are shown for the heparanase gene promoter (C and F), the CD69 gene promoter (D and G), and across the CD69 gene region (E). The names and positions of the primer sets are indicated beneath the graphs. The results represent the mean ± the standard error of 11 independent experiments for panels C to E and 3 independent experiments for panels F and G.