FIG. 6.

H3.3 and H2A.Z changes upon transcriptional activation of CD69 and heparanase. (A to E) ChIP was conducted with Jurkat T cells, MDA cells, and MCF cells with an anti-H3.3 antibody (A, B, and E), an anti-H2A.Z antibody (C to E), or an anti-H3 antibody (E). H3.3 levels are shown across the CD69 gene region (A) and on the heparanase gene promoter (B) in Jurkat T cells left NS or stimulated with PI for the times indicated. Similar data are shown for H2A.Z and the CD69 and heparanase genes in panels C and D, respectively. Panel E depicts the H2A.Z, H3.3, and H3 occupancy in MDA and MCF cells. The names and positions of the primer sets are indicated beneath all of the graphs. Representative data from one of three independent experiments are shown.