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. 2009 Jan 21;29(7):1972–1986. doi: 10.1128/MCB.01590-08

FIG. 8.

FIG. 8.

Deposition patterns of PTMs associated with active transcription during CD69 and heparanase gene induction. (A and B) Acetylated H3K9 (H3K9Ac) ChIP was performed with Jurkat T cells left NS or stimulated with PI for the times indicated. H3K9Ac levels were assessed across the CD69 gene region (A) and on the heparanase gene promoter (B) with the indicated primer sets. Representative data from one of three independent experiments are shown. (C to E) Dimethylated H3K4 (H3K4me2) ChIP was performed with Jurkat T cells left NS or stimulated with PI for the times indicated. H3K4me2 levels were determined on the CD69 gene promoter (C), within the CD69 transcribed region (D), and on the heparanase gene promoter (E) with the indicated primer sets. Data pooled from two independent experiments are shown, with the mean ± the standard error plotted.