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. 2009 Jan 21;29(7):1735–1748. doi: 10.1128/MCB.01483-08

FIG. 1.

FIG. 1.

Dia1, Abi, and Nap1, but not Wave or Arp2/3, are required for cell-cell junctions. (A) A431 cells were transfected with the indicated siRNAs, and after 48 h in the presence of 2 mM calcium-containing media, cells were fixed and stained with anti-β-catenin antibody (red) and counterstained with DAPI (blue). The percentages of cells with β-catenin-positive junctions in randomly selected regions were analyzed. Results represent the means ± SEMs of at least three independent experiments (bar, 10 μm). *, P < 0.01. (B) A431 cells transfected with the indicated siRNAs were lysed, and the cell lysates were analyzed by blotting with the indicated antibodies. (C) A431 cells transfected with Nap1 or Abi1/2 siRNAs showed discontinuous junctions with β-catenin-positive fingerlike extensions between adjacent cells (bar, 10 μm). (D) Vertical sections of A431 cells transfected with Nap1 or Abi1/2 siRNAs. Cells were fixed and stained with anti-β-catenin antibody (bar, 10 μm).