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ER exit sites are not disrupted in the enlarged ER. (A) p31flox/− MEFs infected with Ad-LacZ (Ctrl) or Ad-Cre (cKO) were fixed 4 days after adenovirus treatment and double stained with antibodies against Sec13 (green) and PDI (red). (B) Higher-magnification images of merged images in panel A. Arrows indicate punctate localization of Sec13 along the ER, demonstrating that ER exit sites are not disrupted in p31-deficient MEFs. (A and B) Bars, 5 μm.
