FIG. 9.
Disorganization of the ER caused by p31 deficiency led to ER stress response. (A) p31flox/+ (Ctrl) or p31flox/− (cKO) MEFs infected with Ad-Cre were double stained with antibodies against PDI (red) and CHOP (green). “day3” represents, e.g., samples prepared on day 3 after adenovirus treatment. The arrow indicates a cell with nuclear translocation of CHOP. (B) The ratio of the number of cells showing nuclear translocation of CHOP versus the number of cells showing the aberrant staining pattern of PDI increased in a time-dependent manner in p31-deficient cells. Note that no CHOP nuclear staining was observed at day 0 after Ad-Cre treatment when aberrant ER was already observed. The error bars represent the mean ± the standard error of the mean (n = 3). Bar, 10 μm (A).