Figure 5.

The relative survival rate of the BR2 mutant comparing with E44. (a) Both strains in stationary phase without any stress treatment, (b) both strains were tested with different environmental stress including heat shock (54°C for 3 minutes), alkali (Tris pH = 10.0 for 30 minutes), acid endurance (acetic acid, pH = 2.8 for 20 minutes), high osmolarity challenge (2.4 M NaCl for 1 hour), and oxidative stress (10 μM H₂O₂ for 30 minutes). Gray columns: E44; white columns: BR2. Columns marked with ∗∗ are significantly different (P < .01).