Table 2.

Oligonucleotides used for cloning, sequencing, and making the deletion mutants of ibeR and tnaA genes.

Primers	Sequences of primers	Retained amino acids
primers for ibeR deletion		Total 56 residues
IbeR-S1 (S = SalI)	5′-GATGTCGACGGGCTTTTCGGCGTCA-3′	52 N-terminal residues
IbeR-B1 (B = BamHI)	5′–CGGGATCCAGTGGCGAGGGTCACA-3′	(MDIIIMNKES...)
IbeR-B2 (B = BamHI)	5′-CAGGATCCAAATGTTGAGCATGCAG-3′	4 C-terminal residues
IbeR-X2 (X = XbaI)	5′-CGTCTAGATAAGGGCTAAACATATCG-3′	(...GSKC)
primers for tnaA deletion		Total 56 residues
TN-S1 (S = SalI)	5′-GGGTCGACCAGAGATCTGGCCGGAAT T-3′	21 N-terminal residues
TN-B1 (B = BamHI)	5′-ACGGATCCAATAACACGAATGCGGAACGGTTC-3′	(MKDYVMENFK...)
TN-B2 (B = BamHI)	5′-TTAGATCTTTTAAACATGTGAAAGAGAACGCG-3′	35 C-terminal residues
TN-X2 (X = XbaI)	5′-CCTCTAGATTAGCCAAATTTAGGTAACAC G-3′	(...RHFTAKLKEV)