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. 2009 Mar 17;4(3):e4914. doi: 10.1371/journal.pone.0004914

Figure 4. Shp2 modulates various signaling pathways in mES and hES cells.

Figure 4

(A) LIF/BMP4 signaling in mESCs. mESCs were stimulated by LIF (1,000 units/ml) or BMP4 (25 ng/ml) for 15 mins after overnight starvation. Total cell lysates were immunoblotted with the indicated antibodies. (B) bFGF/BMP4 signaling in hESCs (H14). The indicated antibodies were used to analyze proteins in hESCs stimulated by bFGF (50 ng/ml, 15 mins) or BMP4 (25 ng/ml, 30 min) after 6-hour starvation. (C, D) mESCs (left) or hESCs (right) were treated with BMP4 for 60 mins and total RNAs were analyzed for Id expression levels (Mean±SEM, n = 3). (a, c, e) mESCs; (b, d, f) hESCs.