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. 2009 Mar 23;4(3):e4963. doi: 10.1371/journal.pone.0004963

Figure 3. Sperm from SP hyh mice have a deficient acrosomal reaction.

Figure 3

Sperm from wild type and SP hyh (hyh) mice were collected from the cauda epididymidis, incubated under capacitation conditions for 1 h and stimulated with buffer (control), 10 µM progesterone (Pg) or 10 µM A23187 (A23187) for 15 min at 37°C. The cells were spotted on slides and fixed in ice-cold methanol. Acrosomal status was evaluated in at least 200 sperm by staining with TRITC-PNA. The data represent the mean±SEM of three independent experiments (*, significant differences between same groups for wild type and hyh mice, P<0.001, Student's t test).