Figure 5. Expression of NK cell receptors is altered by activated Notch.

(A) Representative histograms of NK cell receptors on NK cells derived from CD34⁺eGFP⁺ and CD34⁺ICN⁺ cells co-culured with EL08-1D2 stroma and cytokines (Flt3-L, IL-7, c-kit-L, IL-15, IL-3) are shown (gated on CD56⁺CD3⁻ cells). ICN⁺ NK cells demonstrated less NKG2A and KIR expression. (B) Bars represent the average expression of receptors on CD56⁺CD3⁻ NK cells derived from stroma cultured CD34⁺eGFP⁺ (□) and CD34⁺ICN⁺ (■) at day 28 (mean of 4 independent experiments±SEM). (C) CD34⁺eGFP⁺ and CD34⁺ICN⁺ cells were cultured for 28 days on EL08-1D2 with Flt3-L, IL-7, c-kit-L, IL-3 with and without IL-15. In the absence of IL-15, only CD34⁺ICN⁺ population gave rise to NK cells. NKG2A expression on CD34⁺ICN⁺ derived NK cells is compared to eGFP derived NK cells with and without IL-15.