Skip to main content
. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2009 Feb;15(2):183–194. doi: 10.1016/j.bbmt.2008.11.031

Figure 6. Notch 1 can replace stroma in supporting NK cell commitment from CD34+ UCB progenitors.

Figure 6

(A) NK cells derived from CD34+eGFP+ and CD34+ICN+ progenitors were cultured without stroma but with cytokines (Flt3-L, IL-7, c-kit-L, IL-15, IL-3). Absolute numbers of cultured eGFP+(●) and ICN+(▲) derived CD56+CD3− NK cells are shown. Only CD34+ICN+ cells were able to expand without stroma (mean of 3 independent experiments shown). (B) The phenotype of CD34+ICN+ derived stroma free cultured NK cells at day 28 is shown and shows the accumulation of CD56+CD117high CD94lo NKG2Alocells.