Figure 3.

Direct inhibition of T cells by lung epithelial cells (LECs) and, to a lesser extent, via dendritic cells (DCs). (A) Ovalbumin (OVA)-pulsed bone marrow dendritic cells (BM-DC) and carboxy fluoroscein succinimidyl ester (CFSE)-labelled DO11.10 T cells were cultured separately with LA4 cells (LA4 DC, LA4 T cells) or control medium (normal DC, normal T cells) for 24 h. These DC and T cells were then co-cultured in different combinations. (B) Irradiated BM-DC plus antimurine-CD3 antibody were used to induce proliferation of CFSE-labelled splenic CD4+ T cells from naïve BALB/c mice in the presence or absence of LA4 cells. In both experiments, T cell proliferation was measured after 72 h of DC/T cell co-culture. The graphs show mean (SEM) percentages of non-proliferated cells from a representative experiment (six samples per group) of three independent experiments. Significant differences indicated by horizontal bars: *p<0.05, **p<0.01.