Figure 2.

(A) Progress of the selection: the fraction is shown for each generation (round of selection). For the first four rounds a selection time of 60 min was used. In round 5, the selection time was reduced to 5 min. At round 7, the selection time was again decreased to 1 min and maintained until round 9. Starting at round 4, self-cleavage activity was measured at 1, 5 and 60 min. (B) Sequence and hypothetical 2D structure of the selected DNAzyme 9-86. (bold-face A, C and U indicate the position of the modified nucleosides 1, 2 and 3, respectively).