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. 2009 Jan 19;37(5):1638–1649. doi: 10.1093/nar/gkn1070

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Gel images (PAGE 7%) demonstrating the importance of the three modifications. (A) Modified DNAs with dA^imTP, dC^aaTP and dU^gaTP single knockouts. (B) Modified DNAs synthesized with dA^imTP as sole modification and with dA^homTP instead of dA^imTP. As a control, the elongated strands were also cleaved with RNase A to identify anticipated cleavage products that are denoted with asterisks. (C) Structure of dA^homTP, which when added as a triphosphate in lieu the histaminyl dATP is incorporated into strands that manifest <10% activity after 1260 min. Time points were 3, 5, 10, 20, 30, 60 and 1260 min.