Table 2.

Effect of metal cations on the cleavage rate of DNAzyme 9-86^a

Metal	kobs (min−1)	krel
Buffer only	0.1041	1
Mg2+ (5 mM)	0.0580	0.56
Mg2+ (0.5 mM)	0.07752	0.75
Mn2+	0.03807	0.37
Ba2+	0.06509	0.63
Co3+	0.04507	0.43
Ce3+	0.005429	0.05
Co2+	0.004388	0.04
UO22+	0.07837	0.75
Ca2+	0.07629	0.73
Fe3+	0.05431	0.52
Hg2+	n.d.b	n.d.
Eu3+	n.d.	n.d.
Sm3+	n.d.	n.d.
Cu2+	n.d.	n.d.
Yb3+	n.d.	n.d.
Cd2+	n.d.	n.d.
Ni2+	n.d.	n.d.
Zn2+	n.d.	n.d.

^aReaction conditions: 200 mM NaCl, 50 mM cacodylate pH 7.4, 500 μM M²⁺/³⁺, RT.

^bNo cleavage detected.