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. 2009 Feb 10;37(5):e40. doi: 10.1093/nar/gkn1055

Figure 3.

Figure 3.

The mutant Taq and KlenTaq enzymes can tolerate high SYBR Green I concentrations in PCR. A 250-bp target was amplified from 0.5 ng lambda DNA in real time PCR with KlenTaq 10 and Taq 22 mutant enzymes as well as with three w.t. Taq enzymes: Fast Start Taq, Jump Start Taq and AmpliTaq Gold (2 U each enzyme) in the presence of various concentrations of SYBR Green. Serial dilutions of the fluorescent dye, 4×, 2×, 1×, 0.5×, 0.25× and 0.125× were tested along with no dye controls (rightmost lanes). The amplified products were analyzed both in ethidium bromide-stained agarose gel (top panels) and by real-time fluorescence incorporation (background subtracted fluorescence values of amplification and melting curves, middle and bottom panels).