Skip to main content
. 2009 Feb 10;37(5):e40. doi: 10.1093/nar/gkn1055

Figure 5.

Figure 5.

Direct PCR detection of exogenous and soil-born target genes in crude soil samples by the Taq mutants. (a) A 630-bp target of the human CCR5 gene was amplified with the Taq 22 mutant or the w.t. enzymes Fast Start Taq and Jump Start Taq from 5 ng human DNA in the presence of serial dilutions of a crude soil extract. Control reactions contained no soil extract. (b) A 600-bp target of Bacillus rRNA gene was amplified with the KlenTaq 10 mutant, Fast Start Taq or Jump Start Taq, straight from a crude soil extract. The extract was present at the range of 2–16% in the reaction, as indicated. Control reactions (lanes 0+) contained 10 ng DNA purified from the same soil extract. The amplified products were analyzed in a 2% agarose gel stained with ethidium bromide along with DNA standards ladders (M) or by real-time fluorescence detection of SYBR Green I incorporation (b, bottom row). The pink, yellow, blue, green and red curves reflect the amplification (background subtracted fluorescence values) in the controls and in the presence of the four increasing concentrations of soil extract, respectively.