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(A) Coomassie stained SDS-PAGE gel of purified protein preparations. Lane 1, molecular size marker (m; molecular mass indicated in kilo Dalton); lane 2, RAD50/MRE11 complex (RM); lane 3, RAD50/MRE11/NBS1 complex (RMN); lane 4, RAD50/NBS1 complex (RN). (B) Immuno-blotting analysis of purified proteins. Blots containing purified RM, RMN and RN preparations were probed with antibodies directed against RAD50, MRE11 and NBS1, as indicated.
