Figure 5.

DNA tethering by RM (A), RMN (B) and RN (C). Reaction mixtures containing 15 nM of 3.0-kb linear DNA fragment and 25 nM of purified protein were deposited on mica and imaged by tapping mode SFM. The scale bars are 200 nm. Colour represents height from 0 to 3 nm (brown to pink), as shown by the inserts. DNA tethers (triangle), free DNA molecules (star) and DNA molecules involved in DNA tethering (circle) were all quantified as described in ‘Materials and Methods’ section.