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Far-ultraviolet CD and thermal denaturation profiles of homodimeric and monomeric I-CreI (a) and I-MsoI (b). All four curves in both CD and thermal denaturation analyses were generated by best fit analyses for a two-state transition, and had 50% transition temperatures of 66.8 (I-CreI), 64.6 (mCreI), 54.3 (I-MsoI) and 56.2°C (mMsoI), respectively.
