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. 2009 Jan 12;37(5):1521–1528. doi: 10.1093/nar/gkn1073

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Mitochondria of C. reinhardtii do not import plastidial tRNAs. (A) Whole-cell (T), mitochondrial (M) and plastidial (P) RNA fractions separated on polyacrylamide gel and stained with ethidium bromide. Regions corresponding to tRNAs (a), rRNAs (b) and plastidial 3S rRNA (c) are indicated. (B) Hybridization of probes specific to mitochondria-encoded tRNA^Met (Mm), tRNA^Gln (Qm) and tRNA^Trp (Wm) and to mitochondrial 3a rRNA (3am), cytosolic 5.8S rRNA (5.8S), plastidial 5S rRNA (5Sp) to northern blots of whole-cell (T), mitochondrial (M) or plastidial (P) RNAs. (C) Hybridization of probes specific to plastidial tRNA genes to northern blots of mitochondrial (M) and plastidial (P) RNAs. Name of probes is given below each northern experiment (see Supplementary data Table S1 online).