Figure 6.

Histone modifications across the human ATF3 gene locus in LNCaP and Hodgkin L428 cells. (A) ChIP assay was performed in LNCaP, DU145, L428, or DAUDI cells using anti-panacetyl H3, anti-trimethyl H3K4 or anti-trimethyl H3K9 antibodies as in Materials and methods section. The P1 and P2 promoter region of the ATF3 gene and the control GAPDH gene was amplified and analyzed by gel electrophoresis. In L428 and DAUDI cells, the promoter region of the human β-globin gene was also examined. (B) Immunoprecipitated DNA by anti-panacetyl H3 or anti-trimethyl H3K4 antibodies of LNCaP (filled circle, open circle), DU145 (filled traingle, open triangle) cells was measured by quantitative PCR throughout the human ATF3 gene locus, and expressed as percent of the input. Data represent the means of three independent experiments with standard error bars for control IgG (open) and specific antibodies (closed), respectively.