Figure 5.
ITC data obtained at 20°C for the addition of ATMND aliquots (each 15 µl of 175 µM) into the solution containing DNA duplex [1.43 ml of 20 µM, 5′-GCA GCT CCC GXG GTC TCC TCG-3′/3′-CGT CGA GGG CCC CAG AGG AGC-5′, X = AP site (dSpacer), C = target cytosine]. Sample solutions were buffered to pH 7.0 with 10 mM sodium cacodylate, containing 100 mM NaCl and 1.0 mM EDTA. The data were best fitted to a model that assumes a single set of identical binding sites, giving the binding enthalpy (ΔHobs) of −12.8 kcal/mol with a binding stoichiometry (n) of 1.1.