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Fluorescence detection of single-base mutation of PCR products present in (A) sense strand and (B) antisense strand of K-ras gene (107-mer, codon 12). After PCR, the products were analyzed in solutions buffered to pH 7.0 with 100 mM sodium cacodylate containing 1.6 mM EDTA, 50 nM ATMND and 5.0 μM AP site-containing probe DNA. Excitation 350 nm; detection 403 nm. Temperature 5°C.
