. 2009 Jan 9;37(5):1411–1422. doi: 10.1093/nar/gkn1079

Table 2.

Temperature dependence of the observed binding enthalpy (ΔH_ob) and calculated heat capacity change (ΔC_p) for the 1,8-naphthyridine binding to cytosine in the 21-meric AP site-containing DNA duplex

	ΔH_obs (kcal/mol)^a				ΔC_p (cal/mol K)^b
	20°C	15°C	10°C	5°C
AND	−20.5 ± 0.6^*1	−20.0 ± 0.6^*2	−19.1 ± 0.5^*2	−18.2 ± 0.1^*2	−161
ATMND	−12.8 ± 0.7^*2	−11.7 ± 0.5^*2	−10.6 ± 0.1^*1	−9.5 ± 0.2^*2	−217

^aΔH_obs was directly determined by ITC experiments. Errors are the standard deviations obtained from at least three independent measurements at each temperature (^*1 4 times; ^*2 3 times). Sample solutions were buffered to pH 7.0 with 10 mM sodium cacodylate, containing 100 mM NaCl and 1.0 mM EDTA. DNA duplex: 5′-GCA GCT CCC GXG GTC TCC TCG-3′/3′-CGT CGA GGG CCC CAG AGG AGC-5′, X = AP site (dSpacer), C = target cytosine.

^bHeat capacity change calculated from the slope δ(ΔH)/δT obtained by linear least squares fit (cf. Figure 6, r = 0.9906 for AND, r = 0.9999 for ATMND).