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. 2009 Jan 16;37(5):1616–1627. doi: 10.1093/nar/gkp001

Figure 2.

Figure 2.

In vitro translation system prepared from bovine liver mitochondria. (A) Dose–response of poly(Phe) synthesis, as a function of the amount of mitochondrial (open circle) or E. coli (filled circle) ribosomes, using E. coli Phe-tRNA and mt EF-Tu/Ts. The reactions were carried out at 37°C for 8 min. (B) Poly(U)-dependent poly(Phe) synthesis activity of the complete mitochondrial system under the optimized conditions for Phe-tRNA from mitochondria (open circle) and E. coli (filled circle), respectively, as described in the Materials and methods section.