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. 2009 Jan 16;37(5):1616–1627. doi: 10.1093/nar/gkp001

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Codon-dependent ribosome binding assays of bovine mt tRNA^Met variants. (A) Native mt tRNA^Met purified from bovine liver mitochondria. (B) Synthetic mt tRNA^Met with no modified bases. (C) Synthetic mt tRNA^Met with two Ψ's. Oligonucleotides AUG₆ (filled circle), AUA₆ (open circle) and AUC₆ (filled triangle) were used as mRNA. The presented values are the averages of three independent experiments and were reproducible within ± 1.2%.