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. 2009 Mar 25;4(3):e4948. doi: 10.1371/journal.pone.0004948

Figure 5. Characterization of Treg function following MV infection.

Figure 5

(A) Analysis of suppressor activity of Tregs isolated from CD150 mice, inoculated with MV (open symbol) or with medium (full symbol), in cocultures with CD4+CD25− effector T cells from either uninfected CD150 mice (left panel), or infected CD150 mice (right panel) in the presence of irradiated CD4+ T cell–depleted splenic APCs and Con A (3 to 5 pooled mice per group). Proliferation of Tregs from either control or CD150 infected mice in response to Con A was ∼300–600 cpm. The results are shown as the mean percentage of proliferation inhibition in triplicate cultures±SD. Results are representative of three different experiments. (B) Splenocytes isolated from either uninfected (left panel) or MV-infected (right panel) CD150 mice and their nontransgenic littermates (wild type) were stimulated with either irradiated Balb/c or C57Bl/6 splenocytes in MLR in triplicate cultures, as described in Methods. Proliferation is expressed as mean proliferation index±SD and is representative from two independent experiments (* P<0,01, Student t-test).