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. 2009 Apr 3;5(4):e1000359. doi: 10.1371/journal.ppat.1000359

Figure 2. LT modulates cytokine expression by epithelial cells.

Figure 2

(A,B) Beas-2B cells were infected with the B. anthracis strains RP9 and RPLC2 at an MOI of 20∶1 for 3 h in the absence of antibiotics. Cells were then washed and incubated in a medium supplemented with antibiotics in the absence (A) or presence (B) of TNFα. After 24 h incubation, supernatants were collected and IL-8 concentrations were measured. (C,D) Beas-2B cells were pretreated with LT (PA+LF) for 1 h before stimulation with TNFα (10 ng/ml). A fixed concentration of PA (1 µg/ml) and increasing concentrations of LF were used. After 24 h incubation, IL-8 mRNA expression (C) and IL-8 secretion (D) were measured. (E) Beas-2B cells were pretreated with LT (1 µg/ml) for 1 h before stimulation with TNFα (10 ng/ml), and IL-6 concentrations were measured after 24 h incubation. (F) Epithelial cells were isolated from lungs of naive mice and pretreated with LT (1 µg/ml) for 1 h before incubation with LPS (1 µg/ml). KC and IL-6 secretions were measured after 24 h stimulation.