Figure 7. LT impairs KC promoter accessibility.

MLE-15 cells were incubated for 1 h with LT (1 µg/ml) and stimulated with TNFα (10 ng/ml) for an additional 45 min. Cells were fixed with formaldehyde and then chromatin sonicated. The relative recruitment of NF-κB-p65 (p65) and the RNAPII (RNA Polymerase II) ChIP were expressed relative to the non-immun IgG rabbit used as a negative control (A). The H2B and the H3 histone tail modifications ChIP were reported to the H3 ChIP and expressed in percentages (B). Each value was corrected for the recruitment of each antibody on the CD44 exon 2 region used as an unregulated control gene by TNFα and LT. Note that the H2B signal relative to H3 is constant upon treatment and the promoters. It was two times more important than H3, probably because of the relative efficiency of antibodies.