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. 2009 Apr 3;5(4):e1000443. doi: 10.1371/journal.pgen.1000443

Figure 4. Immunohistochemical analysis of HES1 and IP3R3 in developing CVP at P0 and W3.

Figure 4

HES1 immunoreactivity exhibited rather uniform distribution in CVP from P0 animals, whereas a few nuclei showed reduction of HES1 immunoreactivity. The cells with reduced nuclear HES1 immunoreactivity exhibited IP3R3 expression at P0. In W3 animals, most of the cells in the taste buds displayed cytoplasmic localization of HES1, suggesting that it was nonfunctional as a transcription regulator. The very few cells retaining HES1 in the nucleus are indicated by white arrows; these cells did not express IP3R3 or SNAP25. However, some of the cells with reduced HES1 reactivity in the nucleus expressed IP3R3, SNAP25, or blood type H antigen (arrowheads).